Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. High tolerance for contaminants. Quantitation Range. g. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. in the assay in order to reduce waste and still achieve comparable results. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. (The same tips can be used. quant it picogreen dsdna assay kit reagents. We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . Materials & Equipment Required But Not Provided • Pipettors - 5 L - 1200 L. The fluorescence intensity of PicoGreen was measured to determine the. Technical Support Customer Service. 7 Q33231 6 1,000 41. Quant-iT PicoGreen dsDNA Reagent. no. high-range. For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 100 ng/µL of Lamba (λ) DNA, sheared by intensive. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. Quant-iT PicoGreen dsDNA Assay Kit. Got it. Easy to use UV absorbance alternative assay that can be. Unit Size. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. After a 5-minute. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 7. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0. Bioz Stars score: 99/100, based on 1 PubMed citations. Quant-iT PicoGreen dsDNA Reagent. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. 2023-11. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Quantify nucleic acids and proteins using four user-selectable fluorescent. The fusion protein nsp7-nsp8 was generated by inserting a GSGSGS linker sequence. 製品番号（カタログ番号）： P7581. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. Description. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Katalognummer: P11496. • Suitable for use in 96- and 384-well plate formats. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. Dilute from 20xTE stock (supplied in the PicoGreen kit). Picogreen assays are compatible with most microplate readers and fluorometers. Lambda DNA was used to generate the. 05 to consider statistical significance. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. This was possible since, due to the low height of the light ray of only 8. An Agilent 2100 Bioanalyzer (Agilent) was used to compare library quality using a High Sensitivity DNA Kit (Agilent), and the purified library was quantified using a Quant-iT PicoGreen dsDNA assay kit (Invitrogen). ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit. Picogreen assays are compatible with most microplate readers and fluorometers. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. PicoGreen dsDNA Assay Kit. Rapidly detect as little as 0. 2 to 100 ng) Assay Kit were used with a Qubit 3. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. 9 Q33230 9 500 28. The normalized DNA was aliquoted into multiple tubes at 50µL volume. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. 実験してみた – PicoGreenをプレートリーダーで使用してみた. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Picogreen assays are compatible with most microplate readers and fluorometers. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. high-range. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. • Specific for dsDNA in the presence of RNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. yields in a method for direct cycle sequencing of PCR products. Learn how to use the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit to quantitate double-stranded DNA (dsDNA) in solution with a fluorometer or a microplate reader. Begin typing to search, use arrow keys to navigate, Enter to select. Qubit® dsDNA HS Assay Kit. Add µL of the Quant-iT™ PicoGreen® dsDNA Reagent (detailed in Section ) to each sample. Picogreen assays are compatible with most microplate readers and fluorometers. After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. To perform fluorometric quantification of cfDNA quantity Quant-iT PicoGreen dsDNA Assay Kit (Thermofisher), was used, following the manufacturer’s instructions. 995 mL TE for the . • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). • Specific for dsDNA in the presence of RNA. Rapidly detect as little as 0. 500/525. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). no. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Unit Size. Picogreen assays are compatible with most microplate readers and fluorometers. • Suitable for use in 96- and 384-well plate formats. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 2. The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. In addition, the assay failed to detect in vitro generated MPO-DNA complexes when spiked into plasma. The assay is a homogeneous, mix-and-read format where the fluorescence is proportional to analyte present. Picogreen assays are compatible with most microplate readers and fluorometers. Picogreen assay kit (Invitrogen, P7589) a. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. 微孔板读数仪. This protocol is a simplified and This protocol is a simplified and condensed version of the full protocol from the manufacturer. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Product Type. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. • Specific for dsDNA in the presence of RNA. The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. Reagents – The Quant-IT™ PicoGreen® kit was purchased from Thermo Fisher Scientific. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. 99 to $1999. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Price from $9. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Quant-iT PicoGreen dsDNA Assay Kit a. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. The reagent must be completely thawed before using it by bringing it to room temperature. The libraries were sequenced using the Illumina HiSeq 2500. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Per Well Volume 150 uL/well. The original sequencing data. The Quant-iT PicoGreen dsDNA. Quant It Picogreen Dsdna Assay Kit Manual - 114,697 . UV흡광 결과보다 민감도 우수. As little as 2. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 99. Qubit RNA XR Standard can be used with either Qubit RNA XR Assay. This protocol is a simplified and condensed version of. Unit Size. P11495. Product Type. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. An aqueous working solution of PG reagent was freshly prepared as a 1:200 dilution of the concentrated DMSO solution to 1x TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. , QuantIt PicoGreen dsDNA assay). Picogreen assays are compatible with most microplate readers and fluorometers. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). Natural cures with herbs are. 适用于（设备）. ZERO BIAS . Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. Mit dem PicoGreen dsDNA. Bioz Stars score: 86/100, based on 1 PubMed citations. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. • Suitable for use in 96- and 384-well plate formats. ITSkabir). Quant It Picogreen Dsdna Staining Kit, supplied by Thermo Fisher, used in various techniques. BR reagent (Component A) 1. 実験してみた – PicoGreenをプレートリーダーで使用してみた. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. No. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 2–100 ng of DNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Highly selective for dsDNA over ssDNA or RNA. In addition to the Quant-iT™ dsDNA Broad-Range Assay Kit described here, we also offer the Quant-iT™ dsDNA High-Sensitivity Assay Kit (Cat. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. Libraries were sequenced on the Illumina NovaSeq. Picogreen assays are compatible with most microplate readers and fluorometers. USA) and quantity was measured using Qubit 2. Effect of the buffer dilution on the DNA measurement with the Quant-It PicoGreen dsDNA assay. Catalog number: P7581. Product Type. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 50 pg to 2 µg. 2. Figure 2. 3,201 3. 1. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. Quantitation Range. PicoGreen Assay: CHO DNA Concentrate (10 µg/mL), 120 µL D553X Quant-iT PicoGreen dsDNA reagent D003 Solution, 1 x 1. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. 2. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. 2. Quantitation Range. PicoGreen dsDNA quantitation reagents are. The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. • Specific for dsDNA in the presence of RNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Figure 1. Picogreen assays are compatible with most microplate readers and fluorometers. 5mL of TE x 5uL PicoReagent. a) 16. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. PicoGreen dsDNA quantitation reagents are. Serial dilutions were made to give appropriate cell, seeding numbers. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;DNA Quantification Using Quant-iT™ PicoGreen® The Quant-iT™ PicoGreen® assay was tested to overcome limitations of the UV absorbance assay at very low DNA concentrations. 3. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). Unit Size. Prepare only the amount of 1:25 diluted PicoGreen required for that run. 5. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Rapidly detect as little as 0. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. 0 mL to 200 uL to fit a 96-well microplate format. 99. Qubit DNA and RNA assays are available with different quantification ranges. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. Rapidly detect as little as 0. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. The HS (high sensitivity) assays quantitate the lowest levels of analyte, the BR (broad range) assays do so across a wider range, and the RNA XR. 0 fluorometer according to the manufacturer’s protocols; a. For duplicate-well droplet. 5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. Signal measurements of all samples were performed using the EnVision 2105 Multimode Plate Reader (PerkinElmer, Waltham, MA,. Supplier: Invitrogen™ P11496. Product Description. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The range of linearity for DNA concentration measurements. For other nucleic acid methods (RiboGreen, OliGreen), quantified in thePicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. PicoGreen dsDNA quantitation reagents are. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal. QuantiFluor® ONE dye sensitivity range is 0. ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). low-range. . The four reactions per sample were pooled, cleaned up with 1x AMPureXP beads, resuspended in 30 ul EB (10 mM Tris-HCl, pH 8. . Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. PicoGreen dsDNA quantitation reagents are. Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. The DeNovix dsDNA Broad Range Assay is ideal for measuring sample concentrations ranging from 0. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. Table 2 summarizes results of the. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. The following protocol is an adaptation of the Mo-lecular ProbesTM dsDNA Quantitation Reagent and Kits product information sheet. Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Not for use in diagnostic procedures. 0 fluorometer and Quant-iT PicoGreen dsDNA. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. 2. Quantification and normalization of double stranded DNA with minimal set-up time. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. Picogreen assays are compatible with most microplate readers and fluorometers. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Automation of the Quant-iT™ PicoGreen® dsDNA Assay Kit on the Fluent. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. 2023. This protocol is a simplified and condensed. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Reagent Search & Data Services Menu Reagent Search. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitQuant-iT™ PicoGreen® dsDNA Reagent and Kits | 4 Experimental Protoco l DNA Standard Curve 1. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-it Picogreen® dsDNA assay kit (ref P11496) was obtained from Thermofisher scientific. Revised: 10–June–2008 | MP 07581 Table 1. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. For a standard. This assay is designed for use with fluorescence microplate readers. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. The assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 1, page 7) and is accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. . The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. Rapidly detect as little as 0. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. 99. 99. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Unit Size. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Similar. Price from $9. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. • Specific for dsDNA in the presence of RNA. g. quant it picogreen dsdna assay kit , 2023-10. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Add 5uL of PicoGreen reagent for every 1mL of buffer. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Rapidly detect as little as 0. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Rapidly detect as little as 0. Product Type. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 10 x 100 µL. Table 2 summarizes results of the. Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all. 2. 1 ng/μL to 2000 ng/μL, with an extended upper range to 4000 ng/μL. Figure 2. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Unit Size. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. assay. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. A 1:200 dilution of PicoGreen reagent is prepared by adding 10 μl of PicoGreen per 2 mL of 1X TE buffer. Table 3. Quant-iT PicoGreen dsDNA Assay Kit.